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The experimental set up <t>of</t> <t>EMF</t> treatments of PC 12 cell suspensions. Notes: ( A ) The microwave chamber. The temperature probe was placed inside the PC 12 suspension, which was placed on the ceramic pedestal. Insets of PC 12 as visualized under phase contrast microscopy in preparation for EMF exposure. Cells appear to be floating in the suspension in a group of few cells. ( B ) Absorbed power and electric field modeling performed using CST Microwave Studio 3D Electromagnetic Simulation Software; the image depicts the position of the tip of the temperature probe (side and top view); on the right is the <t>Peltier</t> heating stage and the PC 12 suspension was spread on the plate (diameter =65 mm, depth of suspension =0.6 mm). ( C ) Temperature rise in the suspension of PC 12 cells in the presence of EMF radiation. The rise in temperature of the cells in the suspension during EMF treatment was monitored every second for the first 60 seconds. EMF treatments were carried out only for 30 seconds to prevent the overheating of cells. ( D ) Heat profile in the suspension of PC 12 in the absence of EMF radiation. In the Peltier heating graph, the temperature profile during EMF treatments was replicated and the cells were exposed to the same temperature but in the absence of EMF radiation. Abbreviation: EMF, electromagnetic field.
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The experimental set up <t>of</t> <t>EMF</t> treatments of PC 12 cell suspensions. Notes: ( A ) The microwave chamber. The temperature probe was placed inside the PC 12 suspension, which was placed on the ceramic pedestal. Insets of PC 12 as visualized under phase contrast microscopy in preparation for EMF exposure. Cells appear to be floating in the suspension in a group of few cells. ( B ) Absorbed power and electric field modeling performed using CST Microwave Studio 3D Electromagnetic Simulation Software; the image depicts the position of the tip of the temperature probe (side and top view); on the right is the <t>Peltier</t> heating stage and the PC 12 suspension was spread on the plate (diameter =65 mm, depth of suspension =0.6 mm). ( C ) Temperature rise in the suspension of PC 12 cells in the presence of EMF radiation. The rise in temperature of the cells in the suspension during EMF treatment was monitored every second for the first 60 seconds. EMF treatments were carried out only for 30 seconds to prevent the overheating of cells. ( D ) Heat profile in the suspension of PC 12 in the absence of EMF radiation. In the Peltier heating graph, the temperature profile during EMF treatments was replicated and the cells were exposed to the same temperature but in the absence of EMF radiation. Abbreviation: EMF, electromagnetic field.
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The experimental set up of EMF treatments of PC 12 cell suspensions. Notes: ( A ) The microwave chamber. The temperature probe was placed inside the PC 12 suspension, which was placed on the ceramic pedestal. Insets of PC 12 as visualized under phase contrast microscopy in preparation for EMF exposure. Cells appear to be floating in the suspension in a group of few cells. ( B ) Absorbed power and electric field modeling performed using CST Microwave Studio 3D Electromagnetic Simulation Software; the image depicts the position of the tip of the temperature probe (side and top view); on the right is the Peltier heating stage and the PC 12 suspension was spread on the plate (diameter =65 mm, depth of suspension =0.6 mm). ( C ) Temperature rise in the suspension of PC 12 cells in the presence of EMF radiation. The rise in temperature of the cells in the suspension during EMF treatment was monitored every second for the first 60 seconds. EMF treatments were carried out only for 30 seconds to prevent the overheating of cells. ( D ) Heat profile in the suspension of PC 12 in the absence of EMF radiation. In the Peltier heating graph, the temperature profile during EMF treatments was replicated and the cells were exposed to the same temperature but in the absence of EMF radiation. Abbreviation: EMF, electromagnetic field.

Journal: International Journal of Nanomedicine

Article Title: Exposure to high-frequency electromagnetic field triggers rapid uptake of large nanosphere clusters by pheochromocytoma cells

doi: 10.2147/IJN.S183767

Figure Lengend Snippet: The experimental set up of EMF treatments of PC 12 cell suspensions. Notes: ( A ) The microwave chamber. The temperature probe was placed inside the PC 12 suspension, which was placed on the ceramic pedestal. Insets of PC 12 as visualized under phase contrast microscopy in preparation for EMF exposure. Cells appear to be floating in the suspension in a group of few cells. ( B ) Absorbed power and electric field modeling performed using CST Microwave Studio 3D Electromagnetic Simulation Software; the image depicts the position of the tip of the temperature probe (side and top view); on the right is the Peltier heating stage and the PC 12 suspension was spread on the plate (diameter =65 mm, depth of suspension =0.6 mm). ( C ) Temperature rise in the suspension of PC 12 cells in the presence of EMF radiation. The rise in temperature of the cells in the suspension during EMF treatment was monitored every second for the first 60 seconds. EMF treatments were carried out only for 30 seconds to prevent the overheating of cells. ( D ) Heat profile in the suspension of PC 12 in the absence of EMF radiation. In the Peltier heating graph, the temperature profile during EMF treatments was replicated and the cells were exposed to the same temperature but in the absence of EMF radiation. Abbreviation: EMF, electromagnetic field.

Article Snippet: The temperature profile during the EMF exposure was replicated using bulk heat treatment by using the Peltier plate heating/cooling system (TA Instruments, New Castle, DE, USA).

Techniques: Microscopy, Software